Obsada:

• Yo Oizumi Professor Layton
• Maki Horikita Luke Triton
• Saki Aibu Emmy Altava
• Maria Darling Luke Triton / Annie Dretche
• Wayne Forester Curtis O'Donnell
• Sarah Hadland Celia Raidley
• David Holt Marco Brock
• Iemasa Kayumi Oswald Whistler
• Jonathan Keeble Jean Descole
• LiLiCo LiLiCo Annie Dretche
• Christopher Robin Miller Professor Layton
• Kenta Miyake Marco Brock
• Nana Mizuki Janice Quatlane
• Claire Morgan Amelia Ruth
• Sumire Morohoshi Nina
• Jôji Nakata Frederick Bargland
• Mamiko Noto Flora
• Fumiko Orikasa Melina Whistler
• Shiro Saito Inspector Chelmey
• Robbie Stevens Oswald Whistler / Pierre Starbuck
• Emma Tate Janice Quatlane / Emmy Altava
• Megumi Toyoguchi Amelia Ruth
• Atsuro Watabe Jean Descole
• Kôichi Yamadera Pierre Starbuck
• Shôzô Îzuka Curtis O'Donnel
• Hôchû Ôtsuka Inspector Clamp Grosky