Obsada:

• Katharine Hepburn Mary Stuart
• Fredric March Bothwell
• Florence Eldridge Elizabeth Tudor
• Douglas Walton Darnley
• John Carradine Rizzio
• Robert Barrat Morton
• Gavin Muir Leicester
• Ian Keith Moray
• Moroni Olsen John Knox
• William Stack Ruthven
• Ralph Forbes Randolph
• Alan Mowbray Throckmorton
• Frieda Inescort Mary Beaton
• Donald Crisp Huntly
• David Torrence Lindsay
• Molly Lamont Mary Livingstone
• Anita Colby Mary Fleming
• Jean Fenwick Mary Seton
• Lionel Pape Burghley
• Alec Craig Donal
• Mary Gordon Nurse
• Monte Blue Messenger
• Leonard Mudie Maitland
• Brandon Hurst Airan
• Wilfred Lucas Lexington
• D'Arcy Corrigan Kirkcaldy
• Frank Baker Douglas
• Cyril McLaglen Faudoncide
• Doris Lloyd Fisherman's Wife
• Robert Warwick Sir Francis Knollys
• Murray Kinnell Judge
• Lawrence Grant Judge
• Ivan F. Simpson Judge
• Nigel De Brulier Judge
• Barlowe Borland Judge
• Walter Byron Walsingham
• Wyndham Standing Sergeant-at-Arms
• Earle Foxe Earl of Kent
• Paul McAllister du Croche
• Lionel Belmore Fisherman
• Gaston Glass Frenchman
• Neil Fitzgerald Nobleman
• Frank Anthony Man
• John Blood Man
• Al Bridge
• Tommy Bupp Boy in Boat
• David Clyde
• Hallam Cooley
• Jean De Briac Man
• Harvey D'Roulle Foster Man
• Jerry Frank
• Bud Geary
• Douglas Gerrard
• Hilda Grenier Woman
• Winter Hall
• Halliwell Hobbes Man
• Robert Homans Jailer
• Shep Houghton Soldier
• Maxine Jennings Woman
• Jean Kircher Prince James
• Judith Kircher Prince James
• Fred Malatesta Man
• G.L. McDonnell Man
• Wedgwood Nowell Queen Elizabeth's Majordomo
• John Pickard Soldier dueling Bothwell
• Father Raemers Man
• Robert Ryan
• Leslie Sketchley
• Wingate Smith
• Pat Somerset Mary's Majordomo
• Harry Tenbrook One of Queen Mary's Guards
• John Tyke Man
• Billy Watson Fisherman's son
• Bobs Watson Fisherman's Son
• Niles Welch Man